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KMID : 1123920070210030765
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Korean Journal of Oriental Physiology and Pathology
2007 Volume.21 No. 3 p.765 ~ p.770
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Effect of Forsythiae Fructus Exract on the Release of Inflammatory Mediatorinduced by Lipopolysaccharide in RAW 264.7 Macrophage
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You Bok-Jong
Kim Hee-Taek
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Abstract
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Forsythiae fructus has traditionally been used for the treatment of erysipelas, skin rash and acute or chronic inflammatory disorders. The effect of Forsythiae fructus against lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), on mouse RAW 264.7 macrophages. Forsythiae fructus extract suppressed the expression of iNOS, COX-2 and NF-B mRNAs on the lipopolysaccharide-stimulated enhancement in RAW 264.7 macrophages. We examined the expression of iNOS and COX-2 in both mRNA and protein levels to investigate the mechanism by which Forsythiae fructus extract inhibits NO production. Forsythiae fructus extract significantly reduced iNOS, NF-B and PGE, but didn’t inhibit COX-2 expression which was induced by LPS treatment in Raw 264.7 cells. These results suggest that Forsythiae fructus exerts anti-inflammatory effects probably by suppression of the iNOS and NF-B expressions.
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KEYWORD
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Forsythiae fructus, lipopolysaccharide, iNOS, COX-2, NF-B, PGE
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